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dapi solution (1 mg/ml) / 1 ml  (Millipore)


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    Structured Review

    Millipore dapi solution (1 mg/ml) / 1 ml

    Dapi Solution (1 Mg/Ml) / 1 Ml, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dapi+solution(1+mg/dapi+solution++1+mg+ml++++1+ml/pmc10826933-28-0-8
    Average 90 stars, based on 1 article reviews
    dapi solution (1 mg/ml) / 1 ml - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Pregnancy trained decidual NK cells protect pregnancies from harmful Fusobacterium nucleatum infection"

    Article Title: Pregnancy trained decidual NK cells protect pregnancies from harmful Fusobacterium nucleatum infection

    Journal: PLOS Pathogens

    doi: 10.1371/journal.ppat.1011923


    Figure Legend Snippet:

    Techniques Used: Purification, Virus, Recombinant

    Related Articles

    Recombinant:

    Article Title: A pseudoautosomal glycosylation disorder prompts the revision of dolichol biosynthesis.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Calnexin StressGen Biotechnologies SPA-860, RRID: AB_312057 DHRSX Sigma HPA003035, RRID: AB_1080631 FLAG M2 Sigma F3165, RRID: AB_259529 Penta-His Qiagen 34660, RRID: AB_2619735 b-tubulin Thermo Fisher Scientific MA516308, RRID: AB_2537819 LAMP2 antibody Santa-Cruz Biotech SC-18822, RRID: AB_626858 Goat-anti-Mouse antibody DAKO P0447, RRID: AB_2617137 Goat-anti-Rabbit antibody DAKO P0448, RRID:AB_2617138 Alexa 488-labeled goat anti-rabbit IgG antibody Thermo Fisher Scientific A-11034, RRID: AB_2576217 Alexa 568-labeled goat anti-mouse IgG antibody Thermo Fisher Scientific A-11019, RRID: AB_143162 Alexa 488-labeled goat anti-mouse IgG antibody Thermo Fisher Scientific A-11001, RRID: AB_2534069 Alexa 568-labeled goat anti-rabbit IgG antibody Thermo Fisher Scientific A-11011, RRID: AB_143157 Chemicals, reagents, and recombinant proteins Bovine Serum Albumin (BSA) Fraction V Sigma 10735086001 Digitonin Invitrogen (Thermo Fisher Scientific) BN2006 DBPS Cytiva SH30264.01 Fetal Bovine Serum Dutcher S1810 Fetal Bovine Serum BioWest S1400 TurboFectin 8.0 Origene TF81005 Lipofectamine3000 Thermo Fisher Scientific 15292465 Opti-MEM Gibco 11058021 DMEM/F-12 Gibco 21041–025 DMEM/F-12, HEPES Gibco 31330038 IMDM Gibco 12440–053 DMEM Gibco 41966052 Cytiva HyClone Fetal Clone III Serum Cytiva 12319862 0.5 mm glass beads Sigma Aldrich Z250465 DAPI Solution (1 mg/mL) Thermo Fisher Scientific 62248 Saponin Sigma-Aldrich 84510 Triton X-100 Sigma-Aldrich T8787 beta-mercaptoethanol Sigma-Aldrich M3148 Pageruler Plus Prestained Protein Ladder Thermo Fisher Scientific 11832124 DMSO Sigma-Aldrich 317275 Para-formaldehyde Thermo Fisher Scientific 28908 Mowiol 4-88 Sigma-Aldrich 81381 RIPA buffer Custom N/A SuperSignal West Pico PLUS Chemiluminescent Substrate Thermo Fisher Scientific 34579 Puromycin Dihydrochloride Gibco A1113802 Difco Casamino Acids, Vitamin Assay Gibco 16219861 Bacto Yeast Extract Gibco 212750 Bacto Peptone Gibco 16299741 Geneticin Selective Antibiotic Gibco 10131035 (Continued on next page) e1 Cell 187, 1–17.e1–e12, July 11, 2024 .. REAGENT or RESOURCE SOURCE IDENTIFIER Hygromycin B Gibco 10687010 GeneRuler Thermo Fisher Scientific SM1331 PBS Thermo Fisher Scientific 10010023 TBS 10X Euromedex ET220 Versene Gibco 15040033 Methanol Thermo Fisher Scientific 10606652 Methanol (LC-MS grade) Biosolve 136878 Chloroform VWR 22711290 Chloroform (LC-MS grade) Biosolve 34806 trimethylsilyl diazomethane (TMSD) Sigma-Aldrich 362832 NAD+ free acid grade II ROCHE 10127990001 NADH disodium salt ROCHE 10128023001 NADP+ sodium salt hydrate Sigma-Aldrich N0505-1G NADPH tetrasodium salt ROCHE 10102824001 Dolichol Aventi polar lipids 9002000 Polyprenol Aventi polar lipids 9002100 Dolichal This study N/A Polyprenal Aventi polar lipids 9002200 Phosphatidylcholine Sigma-Aldrich P-0378 Phosphatidylethanolamine Sigma-Aldrich P-0503 Glucose Sigma-Aldrich G7021 Magnesium Sulfate Thermo Fisher Scientific A14491.0I Asahipak NH2P-50 4E column Shodex N/A 7-AAD Thermo Fisher Scientific A1310 Carbenicillin VWR J67159.AD Dithiothreitol Thermo Fisher Scientific R0861 Gelred nucleic acid stain Sigma-Aldrich SCT123 T7 DNA Ligase New England Biolabs M0318S CutSmart Buffer New England Biolabs B7204 Agilent 6546 ion funnel mass spectrometer Agilent N/A Agilent 1290 HPLC System Agilent N/A Accucore C30 150 3 2.1 mm column Thermo Fisher Scientific 27826–152130 Isopropopanol Biosolve 162678 Acetonitrile Biosolve 12078 Ammonium formate Biosolve 19878 Formic acid Biosolve 232478 iBlot 2 NC mini Stacks Invitrogen IB23002 ECL WB substrate Thermo Fisher Scientific PIER32106 First-strand cDNA Synthesis Kit Sigma Aldrich GE27-9261-01 DNAse I Sigma Aldrich 10104159001 Trypsin Gibco 25300096 IPTG Thermo Fisher Scientific 15529019 Bolt 4–12% Bis-Tris Plus Gels, 12-well Thermo Fisher Scientific 15324604 Bolt Transfer Buffer (20X)-1 L Thermo Fisher Scientific 15256066 20X Bolt MES SDS Running Buffer (500 mL) Thermo Fisher Scientific 13266499 LDS Sample buffer Thermo Fisher Scientific 11549166 cOmplete(TM), Mini, EDTA-free Protease Thermo Fisher Scientific 11836170001 Trypsin Sigma Aldrich T8003-500MG Trypsin-EDTA 1X in PBS w/o Calcium w/o Magnesium Dominique Dutscher L0940-100 (Continued on next page) ll OPEN ACCESS Cell 187, 1–17.e1–e12, July 11, 2024 e2 Please cite this article in press as: Wilson et al., A pseudoautosomal glycosylation disorder prompts the revision of dolichol biosynthesis, Cell (2024), https://doi.org/10.1016/j.cell.2024.04.041 Article

    Incubation:

    Article Title: Neuronal lineage tracing from progenitors in human cortical organoids reveals mechanisms of neuronal production, diversity, and disease.
    Article Snippet: In brief Bury et al. utilize genetic lineage tracing to determine the progenitor lineage of neurons in human cortical organoids.. They findmost glutamatergic neurons are produced through intermediate progenitors, similar neurons from different progenitor lineages are transcriptionally distinct, and ASDpatient-derived genetic variants alter the proportion and transcriptional profile of lineage-specific groups.

    Article Title: Natural variation in infection specificity of Caenorhabditis briggsae isolates by two RNA viruses
    Article Snippet: .. The next day, they were washed with 1 mL wash solution, resuspended in 0.02% DAPI (Sigma #D9564, 5 mg/mL) in 1 mL wash solution and incubated 30 min at 30°C in the dark. ..

    Flow Cytometry:

    Article Title: Preclinical efficacy of a HER2 synNotch/CEA-CAR combinatorial immunotherapy against colorectal cancer with HER2 amplification.
    Article Snippet: Flow cytometry and cell sorting Adherent cells were washed twice with cold PBS (Sigma-Aldrich), detached with 1 mM PBS-EDTA and resuspended (100,000 cells in 200 mL) in PBS-1% BSA plus 1 mL of anti-HER2 (PE Mouse Anti-Human HER-2/neu Clone NEU24.7) or anti-CEA (APC, CD66abce Antibody, anti-human REAaffinity Clone REA876) antibody for 30 min on ice. .. Cells were then washed with cold PBS, costained with DAPI (1 mL of 1 mg/mL working solution, SigmaAldrich) for 5 min at 4 C and analyzed by flow cytometry (Cyan ADP, Beckman Coulter s.r.l.) using Summit 4.3 software (Dako). ..

    Software:

    Article Title: Preclinical efficacy of a HER2 synNotch/CEA-CAR combinatorial immunotherapy against colorectal cancer with HER2 amplification.
    Article Snippet: Flow cytometry and cell sorting Adherent cells were washed twice with cold PBS (Sigma-Aldrich), detached with 1 mM PBS-EDTA and resuspended (100,000 cells in 200 mL) in PBS-1% BSA plus 1 mL of anti-HER2 (PE Mouse Anti-Human HER-2/neu Clone NEU24.7) or anti-CEA (APC, CD66abce Antibody, anti-human REAaffinity Clone REA876) antibody for 30 min on ice. .. Cells were then washed with cold PBS, costained with DAPI (1 mL of 1 mg/mL working solution, SigmaAldrich) for 5 min at 4 C and analyzed by flow cytometry (Cyan ADP, Beckman Coulter s.r.l.) using Summit 4.3 software (Dako). ..

    other:

    Article Title: Development and Holocrine Secretion of Resin Ducts in Kielmeyera appariciana (Calophyllaceae)
    Article Snippet: For DNA labeling, samples fixed in Karnovsky’s solution were sectioned free-hand and stained with DAPI (4′,6-diamidino-2-phenylindole; 1 mg.mL −1 ) in phosphate-buffered saline (PBS) and 1% Triton X-100 (Sigma, St. Louis, MO, USA), washed in water and mounted in an antifade mounting medium [ ].

    Article Title: Pregnancy trained decidual NK cells protect pregnancies from harmful Fusobacterium nucleatum infection
    Article Snippet: DAPI Solution (1 mg/mL) / 1 mL , Sigma-Aldrich , TS-62248.

    Staining:

    Article Title: Vanillin Promotes Osteoblast Differentiation, Mineral Apposition, and Antioxidant Effects in Pre-Osteoblasts.
    Article Snippet: .. The cells were fixed using a 4% formalin solution, and the nuclei were stained with a 1 mg/mL DAPI solution (Sigma-Aldrich). .. The slides (Thermo Fisher Scientific) were mounted with Fluoromount Aqueous Mounting Medium (Sigma-Aldrich), and the images were captured using an Olympus IX73 inverted microscope (Olympus Corporation, Tokyo, Japan).

    Article Title: Vanillin Promotes Osteoblast Differentiation, Mineral Apposition, and Antioxidant Effects in Pre-Osteoblasts
    Article Snippet: .. The cells were fixed using a 4% formalin solution, and the nuclei were stained with a 1 mg/mL DAPI solution (Sigma-Aldrich). .. The slides (Thermo Fisher Scientific) were mounted with Fluoromount Aqueous Mounting Medium (Sigma-Aldrich), and the images were captured using an Olympus IX73 inverted microscope (Olympus Corporation, Tokyo, Japan).



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    Image Search Results


    Journal: eLife

    Article Title: FABP4-mediated lipid accumulation and lipolysis in tumor-associated macrophages promote breast cancer metastasis

    doi: 10.7554/eLife.101221

    Figure Lengend Snippet:

    Article Snippet: Chemical compound, drug , DAPI solution (1 mg/mL) , Invitrogen , Cat#62248 , 1:1000.

    Techniques: Concentration Assay, Staining, Saline, SYBR Green Assay, Red Blood Cell Lysis, Cell Culture, XF Assay, Reverse Transcription, Polymer, Plasmid Preparation, Blocking Assay, Enzyme-linked Immunosorbent Assay, Sequencing, Transfection, Recombinant, Microarray, Expressing, Derivative Assay, Real-time Polymerase Chain Reaction, Software, Sterility, Migration, Pore Size, Flow Cytometry, Quantitative RT-PCR, Imaging, Microscopy, Transmission Assay, Electron Microscopy

    Journal: PLOS Pathogens

    Article Title: Pregnancy trained decidual NK cells protect pregnancies from harmful Fusobacterium nucleatum infection

    doi: 10.1371/journal.ppat.1011923

    Figure Lengend Snippet:

    Article Snippet: DAPI Solution (1 mg/mL) / 1 mL , Sigma-Aldrich , TS-62248.

    Techniques: Purification, Virus, Recombinant